This platform is located in the CRBC (4th floor), next to medical school. It performs histological analyses on animal and plant tissue samples. The platform was established through financial support from Blaise Pascal University, the European Union (FEDER), the Auvergne regional council, La ligue Contre le Cancer and Canceropôle Lyon Auvergne Rhône Alpes (CLARA)
Missions
The platform is equipped with :
- STP 120 (Microm) tissue processor for dehydration and paraffin inclusion
- EC 350 (Microm) tissue embedding console system to embed tissues in paraffin blocks
- Two HM 340E (Microm) microtomes
- HM 560 (Microm) cryostat
- Myreva SS30 (Microm) automatic slide stainer for dewaxing and HE /TMG staining
- InSitu Pro VSI (Intavis) for automated hybridization in situ and immunohistochemistry on whole-mount tissues or slides
- AxioscanZ1 (Zeiss) slide scanner for fluorescence and brightfield
- AxioImager M2 (Zeiss) microscope with Apotome for fluorescence and brightfield
- AxioObserver 7 (Zeiss) microscope for fluorescence and brightfield with CO2 and temperature-controlled incubation chamber
- MZ7.5 (Leica) stereomicroscopes with 12Mpixels color camera
- X-Clarity (Logos Biosystem) electrophoresis tissue clearing system
- HybEZ oven (ACD) for RNAscopeTM
Access conditions
- Free access for iGReD users and open paid access for externals users
- The platform does not provide full service but delivers training, advice and help for histology analyses
- Access to equipment requires users’ training by platform staff
- After training, access to equipment is subject to reservation of devices on iGReD’s intranet https://grr.gred-clermont.fr/login.php
- Pricing depends on users’ affiliations (iGReD, academia, private sector)
Scientific Director
Technical director
Member
Publications
Loss of SUMO-specific protease 2 causes isolated glucocorticoid deficiency by blocking adrenal cortex zonal transdifferentiation in mice.
Published on 21 Dec 2022 in Nature communications , vol. 13 - pp 7858
Dufour D , Dumontet T , Sahut-Barnola I, Carusi A, Onzon M , Pussard E, Wilmouth JJ , Olabe J , Lucas C , Levasseur A , Damon-Soubeyrand C , Pointud JC , Roucher-Boulez F , Tauveron I , Bossis G, Yeh ET, Breault DT, Val P , Lefrançois-Martinez AM, Martinez A
Loss of LGR4/GPR48 causes severe neonatal salt-wasting due to disrupted WNT signaling altering adrenal zonation.
Published on 20 Dec 2022 in The Journal of clinical investigation
Lucas C , Sauter KS, Steigert M, Mallet D, Wilmouth J Jr , Olabe J , Plotton I, Morel Y, Aeberli D, Wagner F, Clevers H, Pandey AV, Val P , Roucher-Boulez F , Fluck CE
Steroidogenic Factor-1 Lineage Origin of Skin Lesions in Carney Complex Syndrome.
Published on 12 Nov 2022 in The Journal of investigative dermatology , vol. 142 - pp 2949-2957.e9
Sahut-Barnola I , Lefrançois-Martinez AM , Dufour D , Botto JM, Kamilaris C, Faucz FR, Stratakis CA, Val P , Martinez A
Sexually dimorphic activation of innate antitumor immunity prevents adrenocortical carcinoma development.
Published on 14 Oct 2022 in Science advances , vol. 8 - pp eadd0422
Wilmouth JJ Jr , Olabe J , Garcia-Garcia D , Lucas C , Guiton R , Roucher-Boulez F , Dufour D , Damon-Soubeyrand C , Sahut-Barnola I , Pointud JC , Renaud Y , Levasseur A , Tauveron I , Lefrançois-Martinez AM , Martinez A , Val P
Protein kinase A drives paracrine crisis and WNT4-dependent testis tumor in Carney complex.
Published on 01 Dec 2021 in The Journal of clinical investigation , vol. 131
Djari C , Sahut-Barnola I , Septier A , Plotton I, Montanier N , Dufour D , Levasseur A , Wilmouth J Jr , Pointud JC , Faucz FR, Kamilaris C, Lopez AG, Guillou F, Swain A, Vainio SJ, Tauveron I , Val P , Lefebvre H, Stratakis CA, Martinez A , Lefrançois-Martinez AM
Hormonal and spatial control of SUMOylation in the human and mouse adrenal cortex.
Published on 12 Sep 2019 in FASEB journal : official publication of the Federation of American Societies for Experimental Biology , vol. 33 - pp 10218-10230
Dumontet T , Sahut-Barnola I , Dufour D , Lefrançois-Martinez AM , Berthon A , Montanier N , Ragazzon B, Djari C , Pointud JC , Roucher-Boulez F , Batisse-Lignier M , Tauveron I , Bertherat J, Val P , Martinez A
A ZNRF3-dependent Wnt/β-catenin signaling gradient is required for adrenal homeostasis.
Published on 01 Feb 2019 in Genes & development , vol. 33 - pp 209-220
Basham KJ, Rodriguez S , Turcu AF, Lerario AM, Logan CY, Rysztak MR, Gomez-Sanchez CE, Breault DT, Koo BK, Clevers H, Nusse R, Val P , Hammer GD
Steroidogenic differentiation and PKA signaling are programmed by histone methyltransferase EZH2 in the adrenal cortex.
Published on 26 Dec 2018 in Proceedings of the National Academy of Sciences of the United States of America , vol. 115 - pp E12265-E12274
Mathieu M , Drelon C , Rodriguez S , Tabbal H , Septier A , Damon-Soubeyrand C , Dumontet T , Berthon A , Sahut-Barnola I , Djari C , Batisse-Lignier M , Pointud JC , Richard D, Kerdivel G, Calméjane MA, Boeva V, Tauveron I , Lefrançois-Martinez AM , Martinez A , Val P
PKA signaling drives reticularis differentiation and sexually dimorphic adrenal cortex renewal.
Published on 25 Jan 2018 in JCI insight , vol. 3
Dumontet T , Sahut-Barnola I , Septier A , Montanier N , Plotton I, Roucher-Boulez F , Ducros V, Lefrançois-Martinez AM , Pointud JC , Zubair M, Morohashi KI, Breault DT, Val P , Martinez A
P53/Rb inhibition induces metastatic adrenocortical carcinomas in a preclinical transgenic model.
Published on 30 Aug 2017 in Oncogene , vol. 36 - pp 4445-4456
Batisse-Lignier M , Sahut-Barnola I , Tissier F, Dumontet T , Mathieu M , Drelon C , Pointud JC , Damon-Soubeyrand C , Marceau G , Kemeny JL, Bertherat J, Tauveron I , Val P , Martinez A , Lefrançois-Martinez AM
PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development.
Published on 14 Sep 2016 in Nature communications , vol. 7 - pp 12751
Drelon C , Berthon A , Sahut-Barnola I , Mathieu M , Dumontet T , Rodriguez S , Batisse-Lignier M , Tabbal H , Tauveron I , Lefrançois-Martinez AM , Pointud JC , Gomez-Sanchez CE, Vainio S, Shan J, Sacco S, Schedl A, Stratakis CA, Martinez A , Val P
mTOR pathway is activated by PKA in adrenocortical cells and participates in vivo to apoptosis resistance in primary pigmented nodular adrenocortical disease (PPNAD).
Published on 15 Oct 2014 in Human molecular genetics , vol. 23 - pp 5418-28
de Joussineau C , Sahut-Barnola I , Tissier F, Dumontet T , Drelon C , Batisse-Lignier M , Tauveron I , Pointud JC , Lefrançois-Martinez AM , Stratakis CA, Bertherat J, Val P , Martinez A
WNT/β-catenin signalling is activated in aldosterone-producing adenomas and controls aldosterone production.
Published on 15 Feb 2014 in Human molecular genetics , vol. 23 - pp 889-905
Berthon A , Drelon C , Ragazzon B, Boulkroun S, Tissier F, Amar L, Samson-Couterie B, Zennaro MC, Plouin PF, Skah S, Plateroti M, Lefèbvre H, Sahut-Barnola I , Batisse-Lignier M , Assié G, Lefrançois-Martinez AM , Bertherat J, Martinez A , Val P
WT1 maintains adrenal-gonadal primordium identity and marks a population of AGP-like progenitors within the adrenal gland.
Published on 14 Oct 2013 in Developmental cell , vol. 27 - pp 5-18
Bandiera R, Vidal VP, Motamedi FJ, Clarkson M, Sahut-Barnola I , von Gise A, Pu WT, Hohenstein P, Martinez A , Schedl A
Analysis of the role of Igf2 in adrenal tumour development in transgenic mouse models.
Published on 28 Aug 2012 in PloS one , vol. 7 - pp e44171
Drelon C , Berthon A , Ragazzon B, Tissier F, Bandiera R, Sahut-Barnola I, de Joussineau C , Batisse-Lignier M , Lefrançois-Martinez AM, Bertherat J, Martinez A , Val P
Cushing’s syndrome and fetal features resurgence in adrenal cortex-specific Prkar1a knockout mice.
Published on 10 Jun 2010 in PLoS genetics , vol. 6 - pp e1000980
Sahut-Barnola I , de Joussineau C , Val P , Lambert-Langlais S, Damon C, Lefrançois-Martinez AM, Pointud JC , Marceau G , Sapin V , Tissier F, Ragazzon B, Bertherat J, Kirschner LS, Stratakis CA, Martinez A
Constitutive beta-catenin activation induces adrenal hyperplasia and promotes adrenal cancer development.
Published on 15 Apr 2010 in Human molecular genetics , vol. 19 - pp 1561-76
Berthon A , Sahut-Barnola I , Lambert-Langlais S, de Joussineau C , Damon-Soubeyrand C , Louiset E, Taketo MM, Tissier F, Bertherat J, Lefrançois-Martinez AM, Martinez A , Val P
A transgenic mouse line with specific Cre recombinase expression in the adrenal cortex.
Published on 05 Mar 2009 in Molecular and cellular endocrinology , vol. 300 - pp 197-204
Lambert-Langlais S, Val P , Guyot S, Ragazzon B, Sahut-Barnola I , De Haze A , Lefrançois-Martinez AM , Martinez A
