The EVALSEM platform is a service and R&D facility in spermiology. It draws on the expertise acquired within GReD over 30 years of fundamental, clinical and applied research on mammalian spermatozoa. The platform is registered with UCA-Partner (https://partner.uca.fr) for its service offering. EVALSEM is also backed by UCA as a "Business Unit" within the CAI "Clermont Auvergne Innovation" development structure (https://www.clermontauvergneinnovation.com).
The service platform's activities are focused on developing a range of tests to assess the structural and functional qualities of spermatozoa, including an evaluation of sperm nucleus integrity (in particular: fragmentation and oxidation). The platform's R&D missions also include the development of new spermatozoa handling and preservation media, as well as the development of non-invasive therapeutic approaches for the restoration and/or control (immunocontraception) of fertility in mammals.
More specifically, our services cover:
-evaluation of classical sperm parameters (count, morphology, mobility, viability,...)
-assessment of advanced nuclear sperm parameters (nuclear condensation, nuclear fragmentation, nuclear oxidation) and functional parameters (mitochondrial activity, membrane lipid peroxidation, ...)
-evaluation of seminal parameters (pH, Reactive Oxygen Species level, total antioxidant capacity, ...)
-development and optimization of new sperm freezing media for different species.
Our expertise is mainly focused on human, murine and livestock spermatozoa (cattle, goats, pigs, horses, ...). For other species, we listen to your needs and, after optimization, we can decline the tests that would be of interest to you.
Collaborations:
EVALSEM collaborates with established international companies:
MEMPHASYS, Australia
CELLOXESS, USA
ELIANCE, France
Missions
Our equipment:
Sperm Class Analyser” analysis station (Microptic, Spain)”
Microidigit Cool” cryopreservation station (IMV, France)
Timelapse system “GERI3” (Genea Biomdx, Australia)
and via iGReD, access to: “Attune TM NxT” flow cytometer (Thermo Fisher Scientific, France)
Contacts
joel.drevet@uca.fr. 0473407413
pauline.villeneuve@uca.fr. 0473407620
fabrice.saez@uca.fr. 0473407620
Scientific Director
Technicals directors
Members
Publications
Epidemiology of sperm DNA fragmentation in a retrospective cohort of 1191 men.
Published on 06 Jun 2023 in Andrology
Yazdanpanah Ghadikolaei P, Ghaleno LR, Vesali S, Janzamin E, Gilani MAS, Sajadi H, Dizaj AVT, Shahverdi A, Drevet JR , Moghadam Masouleh AA
Serum lipid profile levels and semen quality: new insights and clinical perpectives for male infertility and men’s health
Published on 20 Jul 2023 in Int Urol Nephrol
Andrade G, Iori I, Hsieh MK, Milani G, Zandona PCE, Teixeira TA, Drevet JR , Costa EM, Hallak J
Spermatozoa isolation with Felix™ outperforms conventional density gradient centrifugation preparation in selecting cells with low DNA damage.
Published on 11 Jan 2023 in Andrology
Villeneuve P , Saez F , Hug E , Chorfa A , Guiton R , Schubert B, Force A, Drevet JR
Three-dimensional imaging of vascular development in the mouse epididymis.
Published on 13 Jun 2023 in eLife , vol. 12
Damon-Soubeyrand C , Bongiovanni A, Chorfa A , Goubely C , Pirot N, Pardanaud L, Piboin-Fragner L, Vachias C , Bravard S , Guiton R , Thomas JL, Saez F , Kocer A , Tardivel M, Drevet JR , Henry-Berger J
Viruses, bacteria and parasites: infection of the male genital tract and fertility.
Published on 20 Jul 2023 in Basic and clinical andrology , vol. 33 - pp 19
Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms.
Published on 02 Feb 2022 in Antioxidants (Basel, Switzerland) , vol. 11
Aitken RJ, Drevet JR , Moazamian A , Gharagozloo P
Marijuana Is Associated With a Hormonal Imbalance Among Several Habits Related to Male Infertility: A Retrospective Study.
Published on 17 Feb 2022 in Frontiers in reproductive health , vol. 4 - pp 820451
Teixeira TA, Iori I, Andrade G, Saldiva PHN, Drevet JR , Costa EMF, Hallak J
OXIDATIVE STRESS AND REPRODUCTIVE FUNCTION: Sperm telomeres, oxidative stress, and infertility.
Published on 26 Oct 2022 in Reproduction (Cambridge, England) , vol. 164 - pp F125-F133
Moazamian A , Gharagozloo P, Aitken RJ, Drevet JR
Paroxetine treatment in an animal model of depression improves sperm quality.
Published on 08 Dec 2022 in PloS one , vol. 17 - pp e0271217
Aghajani R, Tavalaee M, Sadeghi N, Razi M, Gharagozloo P, Arbabian M, Drevet JR , Nasr-Esfahani MH
Reactive Oxygen Species and Their Consequences on the Structure and Function of Mammalian Spermatozoa.
Published on 01 Sep 2022 in Antioxidants & redox signaling , vol. 37 - pp 481-500
Drevet JR , Hallak J, Nasr-Esfahani MH, Aitken RJ
Sperm motility in asthenozoospermic semen samples can be improved by incubation in a continuous single culture medium (CSCM®).
Published on 01 Feb 2022 in Systems biology in reproductive medicine , vol. 68 - pp 25-35
Ranéa C, Pariz JR, Drevet JR , Hallak J
Standards in semen examination: publishing reproducible and reliable data based on high-quality methodology.
Published on 31 Oct 2022 in Human reproduction (Oxford, England) , vol. 37 - pp 2497-2502
Björndahl L, Barratt CLR, Mortimer D, Agarwal A, Aitken RJ, Alvarez JG, Aneck-Hahn N, Arver S, Baldi E, Bassas L, Boitrelle F, Bornman R, Carrell DT, Castilla JA, Cerezo Parra G, Check JH, Cuasnicu PS, Darney SP, de Jager C, De Jonge CJ, Drevet JR , Drobnis EZ, Du Plessis SS, Eisenberg ML, Esteves SC, Evgeni EA, Ferlin A, Garrido N, Giwercman A, Goovaerts IGF, Haugen TB, Henkel R, Henningsohn L, Hofmann MC, Hotaling JM, Jedrzejczak P, Jouannet P, Jørgensen N, Kirkman Brown JC, Krausz C, Kurpisz M, Kvist U, Lamb DJ, Levine H, Loveland KL, McLachlan RI, Mahran A, Maree L, Martins da Silva S, Mbizvo MT, Meinhardt A, Menkveld R, Mortimer ST, Moskovtsev S, Muller CH, Munuce MJ, Muratori M, Niederberger C, O'Flaherty C, Oliva R, Ombelet W, Pacey AA, Palladino MA, Ramasamy R, Ramos L, Rives N, Roldan ER, Rothmann S, Sakkas D, Salonia A, Sánchez-Pozo MC, Sapiro R, Schlatt S, Schlegel PN, Schuppe HC, Shah R, Skakkebæk NE, Teerds K, Toskin I, Tournaye H, Turek PJ, van der Horst G, Vazquez-Levin M, Wang C, Wetzels A, Zeginiadou T, Zini A
The NLRP3 inflammasome: molecular activation and regulation in spermatogenesis and male infertility; a systematic review
Published on 30 May 2022 in Basic Clin Androl , vol. 32 - pp 8
Tavalaee M, Rahmani M, Drevet JR , Nasr-Esfahani MH
Vitamin D and calcium, together and separately, play roles in female reproductive performance.
Published on 21 Jun 2022 in Scientific reports , vol. 12 - pp 10470
Safari H, Hajian M, Nasr-Esfahani MH, Forouzanfar M, Drevet JR
Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation.
Published on 21 Oct 2021 in Basic and clinical andrology , vol. 31 - pp 23
Sadeghi N, Tavalaee M, Kiani-Esfahani A, Moazamian A , Gharagozloo P, Aitken RJ, Drevet JR , Nasr-Esfahani MH
Beneficial effects of hypotaurine supplementation in preparation and freezing media on human sperm cryo-capacitation and DNA quality.
Published on 04 Nov 2021 in Basic and clinical andrology , vol. 31 - pp 26
Pons-Rejraji H , Vorilhon S , Difrane A, Dollet S, Bourgne C, Berger M, Chaput L, Pereira B, Bouche C, Drevet JR , Brugnon F
Deferasirox, an Iron-Chelating Agent, Improves Testicular Morphometric and Sperm Functional Parameters in a Rat Model of Varicocele.
Published on 08 Apr 2021 in Oxidative medicine and cellular longevity , vol. 2021 - pp 6698482
Rahmani M, Tavalaee M, Hosseini M, Eskandari A, Shaygannia E , Sadeghi N, Nazem MN, Gharagozloo P, Drevet JR , Nasr-Esfahani MH
Dysplasia of the fibrous sheath with axonemal and centriolar defects combined with lack of mitochondrial activity as associated factors of ICSI failure in primary ciliary dyskinesia syndrome.
Published on 01 May 2021 in International braz j urol : official journal of the Brazilian Society of Urology , vol. 47 - pp 617-626
Pariz JR, Rané C, Drevet J , Hallak J
Field study reveals morphological and neuroendocrine correlates of seasonal breeding in female water voles, Arvicola terrestris.
Published on 15 Sep 2021 in General and comparative endocrinology , vol. 311 - pp 113853
Poissenot K , Moussu C, Chesneau D, Ramadier E, Abi Khalil R, Chorfa A , Chemineau P, Michelin Y, Saez F , Drevet J , Benoit E, Lattard V, Pinot A, Dardente H, Keller M
Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes.
Published on 15 Sep 2021 in International journal of molecular sciences , vol. 22
Chorfa A , Goubely C , Henry-Berger J , Guiton R , Drevet JR , Saez F
Oxidation of Sperm DNA and Male Infertility.
Published on 12 Jan 2021 in Antioxidants (Basel, Switzerland) , vol. 10
Rashki Ghaleno L, Alizadeh A, Drevet JR , Shahverdi A, Valojerdi MR
Serum vitamin D content is associated with semen parameters and serum testosterone levels in men.
Published on 01 Jan 2021 in Asian journal of andrology , vol. 23 - pp 52-58
Ciccone IM, Costa EM, Pariz JR, Teixeira TA, Drevet JR , Gharagozloo P, Aitken RJ, Hallak J
Three-dimensional confocal analysis of chromosome positioning coupled with immunofluorescence in mouse sperm nuclei
Published on 01 Jan 2021 in Methods in molecular biology (Clifton, N.J.) , vol. 2198 - pp 255-268
Champroux A , Goubely C , Henry-Berger J , Drevet JR , Kocer A
Varicocoele and oxidative stress: New perspectives from animal and human studies.
Published on 01 Mar 2021 in Andrology , vol. 9 - pp 546-558
Razi M, Tavalaee M, Sarrafzadeh-Rezaei F, Moazamian A , Gharagozloo P, Drevet JR , Nasr-Eshafani MH
Viral infections and implications for male reproductive health.
Published on 01 Jul 2021 in Asian journal of andrology , vol. 23 - pp 335-347
Teixeira TA, Oliveira YC, Bernardes FS, Kallas EG, Duarte-Neto AN, Esteves SC, Drevet JR , Hallak J
Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?
Published on 13 Aug 2020 in Basic and clinical andrology , vol. 30 - pp 12
Mohammadi Z, Tavalaee M, Gharagozloo P, Drevet JR , Nasr-Esfahani MH
Endoplasmic Reticulum Stress (ER Stress) and Unfolded Protein Response (UPR) Occur in a Rat Varicocele Testis Model.
Published on 29 Jul 2020 in Oxidative medicine and cellular longevity , vol. 2020 - pp 5909306
Hosseini M, Shaygannia E , Rahmani M, Eskandari A, Golsefid AA, Tavalaee M, Gharagozloo P, Drevet JR , Nasr-Esfahani MH
Impact of vitamin D deficiency on mouse sperm structure and function.
Published on 01 Sep 2020 in Andrology , vol. 8 - pp 1442-1455
Shahreza FD, Hajian M, Gharagozloo P, Drevet JR , Nasr-Esfahani MH
Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice.
Published on 01 Nov 2020 in Asian journal of andrology , vol. 22 - pp 590-601
Chu C, Yu L, Henry-Berger J , Ru YF, Kocer A , Champroux A , Li ZT, He M, Xie SS, Ma WB, Ni MJ, Ni ZM, Guo YL, Fei ZL, Gou LT, Liu Q, Sharma S, Zhou Y, Liu MF, Chen CD, Eamens AL, Nixon B, Zhou YC, Drevet JR , Zhang YL
Oxidation of Sperm Nucleus in Mammals: A Physiological Necessity to Some Extent with Adverse Impacts on Oocyte and Offspring.
Published on 23 Jan 2020 in Antioxidants (Basel, Switzerland) , vol. 9
Drevet JR , Aitken RJ
The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword.
Published on 27 Jan 2020 in Antioxidants (Basel, Switzerland) , vol. 9
Aitken RJ, Drevet JR
Impairment of sperm maturation and capacitation due to diet-dependent cholesterol overload.
Published on 01 Sep 2019 in Andrology , vol. 7 - pp 654-661
Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer.
Published on 23 Oct 2019 in Oxidative medicine and cellular longevity , vol. 2019 - pp 6472945
Pariz JR, Ranéa C, Monteiro RAC, Evenson DP, Drevet JR , Hallak J
Oxidative Damage to Sperm DNA: Attack and Defense.
Published on 01 Jan 2019 in Advances in experimental medicine and biology , vol. 1166 - pp 107-117
Drevet JR , Aitken RJ
Paternal impacts on development: identification of genomic regions vulnerable to oxidative DNA damage in human spermatozoa.
Published on 02 Oct 2019 in Human reproduction (Oxford, England) , vol. 34 - pp 1876-1890
Xavier MJ, Nixon B, Roman SD, Scott RJ, Drevet JR , Aitken RJ
The epididymal immune balance: a key to preserving male fertility.
Published on 01 Nov 2019 in Asian journal of andrology , vol. 21 - pp 531-539
A Decade of Exploring the Mammalian Sperm Epigenome: Paternal Epigenetic and Transgenerational Inheritance.
Published on 15 May 2018 in Frontiers in cell and developmental biology , vol. 6 - pp 50
Champroux A , Cocquet J, Henry-Berger J , Drevet JR , Kocer A
Accuracy of human sperm DNA oxidation quantification and threshold determination using an 8-OHdG immuno-detection assay.
Published on 01 Apr 2018 in Human reproduction (Oxford, England) , vol. 33 - pp 553-562
Vorilhon S , Brugnon F , Kocer A , Dollet S, Bourgne C, Berger M, Janny L , Pereira B, Aitken RJ, Moazamian A , Gharagozloo P, Drevet J , Pons-Rejraji H
Epididymal approaches to male contraception.
Published on 06 Nov 2018 in Basic and clinical andrology , vol. 28 - pp 12
Male reproductive health statement (XIIIth international symposium on Spermatology, may 9th-12th 2018, Stockholm, Sweden.
Published on 29 Oct 2018 in Basic and clinical andrology , vol. 28 - pp 13
Levine H, Mohri H, Ekbom A, Ramos L, Parker G, Roldan E, Jovine L, Koelle S, Lindstrand A, Immler S, Mortimer S, Mortimer D, van der Horst G, Ishijima S, Aneck-Hahn N, Baldi E, Menkveld R, Rothmann SA, Giwercman A, Giwercman Y, Holmberg M, Kvist U, Björndahl L, Holmberg R, Arver S, Flanagan J, Drevet JR
A sequence of 28S rRNA-derived small RNAs is enriched in mature sperm and various somatic tissues and possibly associates with inflammation.
Published on 01 Jun 2017 in Journal of molecular cell biology , vol. 9 - pp 256-259
Chu C, Yu L, Wu B, Ma L, Gou LT, He M, Guo Y, Li ZT, Gao W, Shi H, Liu MF, Wang H, Chen CD, Drevet JR , Zhou Y, Zhang Y
Does sperm quality and DNA integrity differ in cryopreserved semen samples from young, adult, and aged Nellore bulls?
Published on 21 Jun 2017 in Basic and clinical andrology , vol. 27 - pp 12
Carreira JT , Trevizan JT, Carvalho IR, Kipper B, Rodrigues LH, Silva C, Perri SHV, Drevet JR , Koivisto MB
Sperm DNA integrity testing: a valuable addition to the tool box of infertility clinicians.
Published on 01 Sep 2017 in Translational andrology and urology , vol. 6 - pp S590-S591
A novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models.
Published on 01 Feb 2016 in Human reproduction (Oxford, England) , vol. 31 - pp 252-62
Gharagozloo P, Gutiérrez-Adán A, Champroux A , Noblanc A , Kocer A , Calle A, Pérez-Cerezales S, Pericuesta E, Polhemus A, Moazamian A , Drevet JR , Aitken RJ
Causes and consequences of oxidative stress in spermatozoa.
Published on 01 Jan 2016 in Reproduction, fertility, and development , vol. 28 - pp 1-10
Aitken RJ, Gibb Z, Baker MA, Drevet J , Gharagozloo P
Mammalian sperm nuclear organization: resiliencies and vulnerabilities.
Published on 21 Dec 2016 in Basic and clinical andrology , vol. 26 - pp 17
Champroux A , Torres-Carreira J , Gharagozloo P, Drevet JR , Kocer A
Sperm DNA damage and assisted reproductive technologies: reasons to be cautious!
Published on 27 Sep 2016 in Basic and clinical andrology , vol. 26 - pp 11
Evaluation of atorvastatin efficacy and toxicity on spermatozoa, accessory glands and gonadal hormones of healthy men: a pilot prospective clinical trial.
Published on 12 Jul 2014 in Reproductive biology and endocrinology : RB&E , vol. 12 - pp 65
Pons-Rejraji H , Brugnon F , Sion B, Maqdasy S , Gouby G, Pereira B, Marceau G , Gremeau AS, Drevet J , Grizard G, Janny L , Tauveron I
Recent knowledge concerning mammalian sperm chromatin organization and its potential weaknesses when facing oxidative challenge.
Published on 01 Apr 2014 in Basic and clinical andrology , vol. 24 - pp 6
DNA oxidative damage in mammalian spermatozoa: where and why is the male nucleus affected?
Published on 01 Dec 2013 in Free radical biology & medicine , vol. 65 - pp 719-723
Noblanc A , Damon-Soubeyrand C , Karrich B, Henry-Berger J , Cadet R , Saez F , Guiton R , Janny L , Pons-Rejraji H , Alvarez JG, Drevet JR , Kocer A
The immunobiology of the mammalian epididymis: the black box is now open!
Published on 02 Oct 2013 in Basic and clinical andrology , vol. 23 - pp 8
[Post-testicular protection of male gametes from oxidative damage. The role of the epididymis].
Published on 30 May 2012 in Medecine sciences : M/S , vol. 28 - pp 519-25
Epididymis response partly compensates for spermatozoa oxidative defects in snGPx4 and GPx5 double mutant mice.
Published on 01 Jan 2012 in PloS one , vol. 7 - pp e38565
Noblanc A , Peltier M, Damon-Soubeyrand C , Kerchkove N, Chabory E, Vernet P, Saez F , Cadet R , Janny L , Pons-Rejraji H , Conrad M, Drevet JR , Kocer A
Dietary cholesterol-induced post-testicular infertility.
Published on 01 Jan 2011 in PloS one , vol. 6 - pp e26966
Ouvrier A, Alves G, Damon-Soubeyrand C , Marceau G , Cadet R , Janny L , Brugnon F , Kocer A , Pommier A, Lobaccaro JM , Drevet JR , Saez F
Glutathione peroxidases at work on epididymal spermatozoa: an example of the dual effect of reactive oxygen species on mammalian male fertilizing ability.
Published on 01 Nov 2011 in Journal of andrology , vol. 32 - pp 641-50
Noblanc A , Kocer A , Chabory E, Vernet P, Saez F , Cadet R , Conrad M, Drevet JR
Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity.
Published on 30 Apr 2010 in Journal of animal science , vol. 88 - pp 1321-31
Chabory E, Damon C, Lenoir A , Henry-Berger J , Vernet P, Cadet R , Saez F , Drevet JR
[Role of reactive oxygen species (ROS) on human spermatozoa and male infertility].
Published on 30 Jun 2009 in Gynecologie, obstetrique & fertilite , vol. 37 - pp 529-35
Pons-Rejraji H , Sion B, Saez F , Brugnon F , Janny L , Grizard G
Epididymis seleno-independent glutathione peroxidase 5 maintains sperm DNA integrity in mice.
Published on 30 Jul 2009 in The Journal of clinical investigation , vol. 119 - pp 2074-85
Chabory E, Damon C, Lenoir A , Kauselmann G, Kern H, Zevnik B, Garrel C, Saez F , Cadet R , Henry-Berger J , Schoor M, Gottwald U, Habenicht U, Drevet JR , Vernet P
GPX5, the selenium-independent glutathione peroxidase-encoding single copy gene is differentially expressed in mouse epididymis.
Published on 01 Jan 2008 in Reproduction, fertility, and development , vol. 20 - pp 615-25
Zhang T, Chabory E, Britan A, Grignard E, Pitiot O, Saez F , Cadet R , Henry-Berger J , Vernet P, Drevet JR
Identification of sperm antigens as a first step towards the generation of a contraceptive vaccine to decrease fossorial water vole Arvicola terrestris Scherman proliferations.
Published on 15 Sep 2007 in Theriogenology , vol. 68 - pp 779-95
Lipid remodeling of murine epididymosomes and spermatozoa during epididymal maturation.
Published on 30 Jun 2006 in Biology of reproduction , vol. 74 - pp 1104-13
Rejraji H, Sion B, Prensier G, Carreras M, Motta C, Frenoux JM, Vericel E, Grizard G, Vernet P, Drevet JR
The antioxidant glutathione peroxidase family and spermatozoa: a complex story.
Published on 16 May 2006 in Molecular and cellular endocrinology , vol. 250 - pp 70-9
