Bertille MONTIBUS
Post-doctorant
MONTIBUS
Bertille
Post-doctorant
Post-Doctorant
Resume
Since 2024 : Post-doctoral fellow (iSite), Université Clermont Auvergne, iGReD, France
2018 - 2024 : Post-doctoral reasearch associate, King's College London, UK
2016 - 2018 : Post-doctoral research associate, Cambridge University, UK
2012 - 2016 : Doctorat, Université d'Auvergne, France
Fundings
2024-2027 : I-Site Clermont Auvergne Project, CAP 20-25 Fellowship
2021 : “Innovation Fund”, King’s College London
2020 : “Genome Editing Mice for Medicine (GEMM) call”, MRC Harwell
2015 : 4th year PhD, Fondation pour la Recherche Médicale
Publications (outside iGReD)
2024
Montibus B*†, Cain, JA*, Martinez-Nunez RT, & Oakey RJ†. (2024). “Global identification of mammalian host and nested gene pairs reveal tissue-specific transcriptional interplay.” Genome research, 34(12), 2163–2175. https://doi.org/10.1101/gr.279430.124 ; * Contribute equally †Co-corresponding authors
Montibus B*, Ragheb R*, Diamanti E, Dunn SJ, Reynolds N, Hendrich B. “The Nucleosome Remodelling and Deacetylation complex coordinates the transcriptional response to lineage commitment in pluripotent cells.” Biology open, 13(1), bio060101. https://doi.org/10.1242/bio.060101; * Contribute equally.
Brempou D, Montibus B, Izatt L, Andoniadou CL, & Oakey RJ. (2024). Using parenclitic networks on phaeochromocytoma and paraganglioma tumours provides novel insights on global DNA methylation. Scientific reports, 14(1), 29958. https://doi.org/10.1038/s41598-024-81486-9
Lando D, Ma X, Cao Y, Jartseva A, Stevens TJ, Boucher W, Reynolds N, Montibus B, Hall D, Lackner A, Ragheb R, Leeb M, Hendrich BD, & Laue ED. (2024). “Enhancer-promoter interactions are reconfigured through the formation of long-range multiway hubs as mouse ES cells exit pluripotency.” Molecular cell, 84(8), 1406–1421.e8. https://doi.org/10.1016/j.molcel.2024.02.015
2022
Cain JA, Montibus B, Oakey RJ. “Intragenic CpG Islands and Their Impact on Gene Regulation.” Front Cell Dev Biol. 2022;10:832348. doi: 10.3389/fcell.2022.832348
2021
Contreras Castillo S, Montibus B, Rocha A, Duke W, von Meyenn F, McLornan D, Harrison C, Mullally A, Schulz R, Oakey RJ. “Hydroxycarbamide effects on DNA methylation and gene expression in myeloproliferative neoplasms.” Genome Res. 2021 Aug;31(8):1381-1394. doi: 10.1101/gr.270066.120
Prickett AR, Montibus B, Barkas N, Amante SM, Franco MM, Cowley M, Puszyk W, Shannon MF, Irving MD, Madon-Simon M, Ward A, Schulz R, Baldwin HS, Oakey RJ. “Imprinted gene expression and function of the Dopa Decarboxylase gene in the developing heart.” Front Cell Dev Biol. 2021 Jun 22;9:676543. doi: 10.3389/fcell.2021.676543
2020
Amante SM, Montibus B, Cowley M, Barkas N, Setiadi J, Saadeh H, Giemza J, Contreras-Castillo S, Fleischanderl K, Schulz R, Oakey RJ. “Transcription of intragenic CpG islands influences spatiotemporal host gene pre-mRNA processing.” Nucleic Acids Res. 2020 Sep 4;48(15):8349-8359. doi: 10.1093/nar/gkaa556
Prouzet-Mauléon V, Montibus B, Chauveau A, Hautin M, Migeon M, Ka C, Laharanne E, Bidet A, Corcos L, Lippert E. “A novel thrombopoietin (THPO) mutation altering mRNA splicing in a case of familial thrombocytosis.” Br J Haematol. 2020 Jul;190(2):e104-e107. doi: 10.1111/bjh.16742
2016
Binamé F, Bidaud-Meynard A, Magnan L, Piquet L, Montibus B, Chabadel A, Saltel F, Lagrée V, Moreau V. “Cancer-associated mutations in the protrusion-targeting region of p190RhoGAP impact tumor cell migration.” J Cell Biol. 2016 Sep 26;214(7):859-73. doi: 10.1083/jcb.201601063.
Research
Unravelling the function of the H13/Mcts2 locus to evaluate its contribution to imprinting disorders.
Members of the group involved in the project : Catherine Barrière (Lecturer), Aurélien Juven (PhD student)
Genomic imprinting is a crucial developmental process that results in the monoallelic expression of genes based on their parental origin. Disruptions in this tightly regulated process, including aberrant expression of imprinted genes, lead to imprinting disorders (IDs)—a group of rare congenital conditions affecting growth and neurodevelopment. Identifying the genetic causes and pathophysiological mechanisms underlying these disorders remains challenging.
The Mulchandani–Bhoj–Conlin syndrome (MBCS) exemplifies this complexity. MBCS is characterized by fetal and postnatal growth restriction, short stature, feeding difficulties leading to failure to thrive, and, in some cases, hypotonia, skeletal anomalies, strabismus, macrocephaly, and developmental delay. MBCS has been associated with the inheritance of the two copies of chromosome 20 from the mother, resulting in aberrant expression of four imprinted loci: NNAT, LMBT3L, GNAS, and HM13/MCTS2. However, the specific contributions of these loci to the diverse clinical features of MBCS remain poorly understood.
Building on growing evidence and my preliminary research, we hypothesize that altered expression of the HM13/MCTS2 locus plays a key role in MBCS pathogenesis and that additional, yet unidentified, molecular mechanisms in the region may be involved. This underscores the need for further investigation into the functions of these poorly characterized imprinted genes.
To address this, our project will explore the regulatory mechanisms and functional roles of HM13/MCTS2 using a combination of phenotypic analysis, molecular studies, and mouse models. By assessing allele-specific expression and isoform regulation at both transcriptional and translational levels, this research will provide deeper insights into the developmental roles of imprinted genes and the consequences of their dysregulation in disease.
Publications
Biallelic non-productive enhancer-promoter interactions precede imprinted expression of Kcnk9 during mouse neural commitment.
Published on 30 Jan 2024 in HGG advances , vol. 5 - pp 100271
Rengifo Rojas C , Cercy J , Perillous S , Gonthier-Guéret C, Montibus B , Maupetit-Méhouas S, Espinadel A , Dupré M , Hong CC, Hata K, Nakabayashi K, Plagge A, Bouschet T, Arnaud P , Vaillant I , Court F
The Long Non-Coding RNA HOXA-AS2 Promotes Proliferation of Glioma Stem Cells and Modulates Their Inflammation Pathway Mainly through Post-Transcriptional Regulation.
Published on 25 Apr 2022 in International journal of molecular sciences , vol. 23
Le Boiteux E , Guichet PO, Masliantsev K, Montibus B , Vaurs-Barriere C, Gonthier-Gueret C, Chautard E, Verrelle P, Karayan-Tapon L, Fogli A , Court F , Arnaud P
TET3 controls the expression of the H3K27me3 demethylase Kdm6b during neural commitment.
Published on 30 Jan 2021 in Cellular and molecular life sciences : CMLS , vol. 78 - pp 757-768
Montibus B , Cercy J , Bouschet T, Charras A, Maupetit-Méhouas S , Nury D , Gonthier-Guéret C, Chauveau S , Allegre N , Chariau C, Hong CC, Vaillant I , Marques CJ, Court F , Arnaud P
Imprinting control regions (ICRs) are marked by mono-allelic bivalent chromatin when transcriptionally inactive.
Published on 29 Jan 2016 in Nucleic acids research , vol. 44 - pp 621-35
Maupetit-Méhouas S , Montibus B , Nury D , Tayama C, Wassef M, Kota SK, Fogli A , Cerqueira Campos F , Hata K, Feil R, Margueron R, Nakabayashi K, Court F , Arnaud P
